Composite

Part:BBa_K727009

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM12_AUC_Turkey   (2012-06-16)


Fish Odor Generator-Component Two

MMT (monomethylamine transferase) catalyses the first reaction of TMA (trimethylamine) synthesis. TMA is the major cause of unpleasant fish odor. DMT (dimethylamine transferase) takes part in the second reaction of the production of TMA (trimethylamine) which is the major cause of unpleasant fish odor. The promoter is attached into the part with the aim of easier regulation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1045
    Illegal suffix found in sequence at 2439
    Illegal EcoRI site found at 2460
    Illegal XbaI site found at 2475
    Illegal SpeI site found at 3882
    Illegal PstI site found at 3896
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 2460
    Illegal SpeI site found at 2440
    Illegal SpeI site found at 3882
    Illegal PstI site found at 2454
    Illegal PstI site found at 3896
    Illegal NotI site found at 1051
    Illegal NotI site found at 2447
    Illegal NotI site found at 2466
    Illegal NotI site found at 3889
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1045
    Illegal EcoRI site found at 2460
    Illegal BglII site found at 2420
    Illegal BglII site found at 2584
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1045
    Illegal suffix found in sequence at 2440
    Illegal EcoRI site found at 2460
    Illegal XbaI site found at 2475
    Illegal SpeI site found at 3882
    Illegal PstI site found at 3896
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1045
    Illegal EcoRI site found at 2460
    Illegal XbaI site found at 1060
    Illegal XbaI site found at 2475
    Illegal SpeI site found at 2440
    Illegal SpeI site found at 3882
    Illegal PstI site found at 2454
    Illegal PstI site found at 3896
    Illegal AgeI site found at 1383
    Illegal AgeI site found at 1458
    Illegal AgeI site found at 2199
    Illegal AgeI site found at 2845
    Illegal AgeI site found at 2854
    Illegal AgeI site found at 3605
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
n/aFish Odor Generator-Component Two